Volume 5, Issue 1, March 2020, Page: 1-10
Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope
Afrina Khan Piya, Department of Mechanical Engineering, Nagaoka University of Technology, Nagaoka City, Japan
Munshi Muhammad Raihan, Department of Mechanical Engineering, Nagaoka University of Technology, Nagaoka City, Japan
Md Alamgir Hossain, Department of Mechanical Engineering, Military Institute of Science and Technology, Dhaka, Bangladesh
Received: Oct. 26, 2019;       Accepted: Nov. 29, 2019;       Published: Mar. 23, 2020
DOI: 10.11648/j.aas.20200501.11      View  271      Downloads  29
Abstract
Atomic force microscopy (AFM) is a great scientific invention that can visualize cell morphology in aqueous environment and provide information to investigate cell biomechanics at a high spatial resolution in a controlled environment with force sensitivity. Contemporary AFM techniques permit solving a number of problems of cell biomechanics due to synchronized evaluation of the local mechanical properties. For characterizing mechanical properties force spectroscopy is used that provides information on cellular structures including cytoskeleton structure and morphology. For the success of biomedical implant, the most crucial factor is biocompatibility and osteo-conductivity of the implant material that can be characterized by the change in mechanical properties of cellular filaments and nucleus determined by exploiting atomic force microscope techniques. Hydroxyapatite is a bioactive material in bio-ceramics, hence used for fillers, bone grafts and metallic implant coating. Recently developed HAp/amino acid fluorescent complexes could be a significant candidate to be used for dental implant, had shown antibacterial properties with visible light irradiation. This study aims at revealing murine osteoblasts cell (MC3T3) adhesion behavior on the HAp coating and HAp/amino acid complexes. The AFM revealed that no significant changes were observed in mechanical properties of the osteoblasts cells when adhered on electrochemically deposited HAp coating and HAp/amino acid ligands complex coating. SEM and EDX analysis revealed cell morphology were identical for HAp and HAp/amino acid ligands complex coating. Such characteristics are desirable for the success of implant biomaterial coating that can preserve both antibacterial property and cell adhesion behavior.
Keywords
Dental Implant, Antibacterial Property, HAp/Amino Acid Complex, Atomic Force Microscopy (AFM), Cell Elasticity, CIP, Cytoskeleton Dynamics, Biomaterials
To cite this article
Afrina Khan Piya, Munshi Muhammad Raihan, Md Alamgir Hossain, Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope, Advances in Applied Sciences. Vol. 5, No. 1, 2020, pp. 1-10. doi: 10.11648/j.aas.20200501.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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